MS12 [C57BL/6] Developed by Shogo Endo, Tokyo Metropolitan Institute of Gerontology. MS12 ES cells derived from C57BL/6 were used to generate the mutant mice. C57BL/6J background. G-substrate KO, G-substrate KO G-substrate KO, G-substrate KO true 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. U S A, 106, 3525-3530 (2009).<br>地方独立行政法人東京都健康長寿医療センター指定のMTAの締結が必要。 C（3〜6か月） 遠藤　昌吾 Fluorescent Proteins/lacZ System bovine tau cDNA, jellyfish GFP derivative (AFAP) cDNA, phage P1 loxP site, mouse Gsbs (Ppp1r17) genomic DNA In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 106, 3525-3530 (2009).Required the closing of MTA with Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology. <a href='https://brc.riken.jp/mus/pcr06468'>Genotyping protocol -PCR-</a> 東京都健康長寿医療センター研究所・遠藤昌吾先生。MS12 ES細胞（C57BL/6由来）を用いて作出。C57BL/6J背景。 C57BL/6J-Ppp1r17<tm1Nkz> C57BL/6J-Ppp1r17<tm1Nkz> G-substrate (Ppp1r17) gene knockout mice. Exon 2 was replaced with a tau-AFAP-PGK-neo cassette. The PGK-neo was subsequently removed via CAG-cre mediated recombination. Homozygous mutant mice exhibit loss of long-term OKR adaptation and temporary loss of cerebellar LTD in early developmental stage. No obvious ataxia or abnormal movement. Endou, Syogo G-substrate (Ppp1r17) 遺伝子のノックアウトマウス。エクソン2をtau-AFAP-PGK-neoカセットで置換。PGK-neoカセットはpCAG-Creにより除去済み。PKGの基質であるG-substrateは小脳プルキンエ細胞に極めて高濃度に存在する。ホモマウスは長期OKR適応消失、早期発達段階での一時的な小脳LTDの消失を呈する。目立った行動の異常は認められない。 RBRC06468 C (3-6 months) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> Cre/loxP system Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Neurobiology Research